What is an Antibody? Antibodies 101

What is an Antibody? Antibodies 101 What is an antibody? Antibodies are large Y-shaped proteins. They are recruited by the immune system to identify and neutralize foreign objects like bacteria and viruses. Humoral immune system Each antibody has a unique target on the invading organism. This antigen is like a key that helps the antibody[…]

Secondary Antibody: How to Choose

Secondary Antibody: How to Choose With so many antibodies on the market, this article “Secondary Antibody: How to Choose” will help a scientist better understand secondary antibodies and give the tools to  choose the best antibody. The main function of the secondary antibody is to bind to the primary antibody. For example, if the primary[…]

Immunoassay Protocols-ELISA’s, Westerns and More

Immunoassay Protocols-ELISA’s, Westerns and More Below are Immunoassay Protocols. Click on the immunoassay below to find the appropriate protocol: ELISA Western Blot Immunohistochemistry (Frozen Section) Immunohistochemistry (Paraffin Sections) Immunoprecipitation     ImmunoReagents, Inc. is a leading global manufacturer of quality antibodies and reagents used in pharmaceutical research, life science research and in vitro diagnostics. Product[…]

Lab Tips and Tricks

Lab Tips and Tricks This is a list of lab tips and tricks that the staff at ImmunoReagents has collected over decades of working in a lab. These are random thoughts and tips and we will continually add to the list as we think of new ones. 15 ml and 50 ml conical cylinders mix[…]

Video Tutorials

Video Tutorials   ImmunoReagents, Inc. is a leading global manufacturer of quality antibodies and reagents used in pharmaceutical research, life science research and in vitro diagnostics. Product offerings include a wide range of immunochemistry reagents such as purified immunoglobulins, primary antibodies, and secondary antibodies covering a broad spectrum of immunoglobulins from various species. ImmunoReagents also[…]

Conjugated Secondary Antibody: A Guide

Conjugated Secondary Antibody: A Guide by: ImmunoReagents University (IRU) Conjugated Secondary Antibody: A Guide is an article designed to help you learn and select the appropriate conjugated secondary antibody. Secondary antibodies are conjugated (AKA-Labeled) to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins.  At times the choices seem overwhelming, but this guide should[…]

Serum Blocking Buffers: ELISA’s/Westerns

Serum Blocking Buffers: ELISA’s/Westerns Serum Blocking Buffers are used frequently in immunoassays and are a critical step. Blocking “fills-in” the unoccupied spaces of the solid phase that are not occupied by immobilized proteins.  Blocking prevents non-specific binding of the antibodies. Without blocking agents, the antibodies could bind without specificity and lead to false signaling and/or[…]

Antibody Buffers and Formulations

Antibody Buffers and Formulations

Antibody Buffers and Formulations Some common antibody buffers and formulations: Phosphate Buffered Saline (PBS)– Dissolve 8.0 grams of NaCl, 0.2 grams of KCl, 1.44 grams of Sodium Phosphate, and 0.24 grams of Potassium Phosphate in 800ml of Deionized (DI) Water. Adjust the pH to 7.2. Bring the final volume to 1 Liter.   Tris-Buffered Saline (TBS)–[…]

Publications

Publications DNAzyme-functionalized gold–palladium hybrid nanostructures for triple signal amplification of impedimetric immunosensor L Hou, Z Gao, M Xu, X Cao, X Wu, G Chen… – Biosensors and …, 2014 – Elsevier … (Cambridge, MA). Polyclonal goat anti-human PSA antibody (designated as Ab 2 ) was purchased from ImmunoReagents, Inc. (Raleigh, NC). PSA standards were obtained[…]

How to Store Antibodies

How to Store Antibodies General Storage Tips: This article describes how to store antibodies. Antibodies, like most proteins, do not like to be frozen and thawed multiple times.  *IR Tip: Avoid repetitive freezing and thawing of your sample. This is called a freeze-thaw cycle. The best way to store your antibody is to keep a high protein[…]