Publications

Publications

DNAzyme-functionalized gold–palladium hybrid nanostructures for triple signal amplification of impedimetric immunosensor

L Hou, Z Gao, M Xu, X Cao, X Wu, G Chen… – Biosensors and …, 2014 – Elsevier

(Cambridge, MA). Polyclonal goat anti-human PSA antibody (designated as Ab 2 ) was purchased
from ImmunoReagents, Inc. (Raleigh, NC). PSA standards were obtained from Biocell Biotechnol.
Co., Ltd. (Zhengzhou, China). Hemin was purchased from Tokyo Chem. Inc.

Stat3 pathway correlates with the roles of leptin in mouse liver fibrosis and sterol regulatory element binding protein-1c expression of rat hepatic stellate cells

W Zhang, M Niu, K Yan, X Zhai, Q Zhou, L Zhang… – The international journal …, 2013 – Elsevier

and activated HSCs (Cassiman et al., 1999), followed by incubation with DyLight594-conjugated
secondary antibody (diluted 1:500, ImmunoReagents, Inc., Raleigh, USA) and DyLight488-
conjugated secondary antibody (diluted 1:500, ImmunoReagents, Inc., Raleigh, USA).

URG4 upregulation is associated with tumor growth and poor survival in epithelial ovarian cancer

W Li, N Zhou – Archives of gynecology and obstetrics, 2012 – Springer

Then, the specimens were briefly washed in PBS and incubated at room temperature with
goat anti-rabbit IgG (GtxRb-003-E, ImmunoReagents, Inc., Raleigh, NC) or goat anti-mouse
IgG (GtxMu-003- F2BIO, ImmunoReagents, Inc., Raleigh, NC). After

Curcumin regulates delta-like homolog 1 expression in activated hepatic stellate cell

J Qiu, Q Zhou, X Zhai, X Jia, Y Zhou – European journal of pharmacology, 2014 – Elsevier

USA) and primary antibody against synaptophysin (SYP, diluted 1:10, Abcam), a marker for
quiescent and activated HSCs (Cassiman et al., 1999), followed by incubation with
DyLight594-conjugated secondary antibody (diluted 1:500, ImmunoReagents, Inc. Raleigh, USA)

GATA binding protein 2 mediates leptin inhibition of PPARγ1 expression in hepatic stellate cells and contributes to hepatic stellate cell activation

Q Zhou, W Guan, H Qiao, Y Cheng, Z Li, X Zhai… – … et Biophysica Acta (BBA …, 2014 – Elsevier

MA, USA), a marker for quiescent and activated HSCs [19], followed by incubation with
DyLight594-conjugated secondary antibody (1:500, ImmunoReagents, Inc., Raleigh, USA) and
DyLight488-conjugated secondary antibody (1:500, ImmunoReagents, Inc., Raleigh, USA).

Activin/Nodal Signaling Switches the Terminal Fate of Human Embryonic Stem Cell-derived Trophoblasts

P Sarkar, SM Randall, TS Collier, A Nero… – Journal of Biological …, 2015 – ASBMB

Cells were blocked in 1X PBS (Sigma-Aldrich) with 5% BSA (AMRESCO, Solon, Ohio), 0.1
mg/ml human IgG (Immunoreagents Inc., Raleigh, NC) and 0.3% Triton X-100, and incubated
overnight with the primary antibody diluted in blocking buffer.

Hydrogen sulfide-induced enhancement of gastric fundus smooth muscle tone mediated by voltage-dependent potassium and calcium channels in mice

XM Meng, X Huang, CM Zhang, DH Liu, HL Lu, WX Xu… – wjgnet.com

the cells were incubated at room temperature with Alexa Fluor 488-conjugated goat anti-mouse
IgG (1:100, Jackson Immuno Research, West Grove, PA, United States) mixed with Dylight
594-conjugated goat anti-rabbit IgG (1:100, ImmunoReagents Inc, Raleigh, NC, United

The methyl donor S-adenosylmethionine potentiates doxorubicin effects on apoptosis of hormone-dependent breast cancer cell lines

CP Ilisso, M Castellano, S Zappavigna, A Lombardi… – Endocrine, 2015 – Springer

All other antibodies were obtained from ImmunoReagents, Inc (Raleigh, NC, USA). CD95-FITC
and FASL were purchased from Gibco (Grand Island, NY). All buffers and solutions were prepared
with ultra-high quality water. All reagents were of the purest commercial grade.

Different regulatory effects of hydrogen sulfide and nitric oxide on gastric motility in mice

X Huang, XM Meng, DH Liu, YS Wu, X Guo… – European journal of …, 2013 – Elsevier

washing, the cells were incubated at room temperature with Alexa Fluor 488-conjugated goat
anti-mouse IgG (1:100, Jackson Immuno Research, West Grove, PA, USA) mixed with DyLight
594-conjugated goat anti-rabbit IgG (1:100, ImmunoReagents Inc, Raleigh, NC, USA) for

Urchin-like (gold CORE)@(platinum shell) nanohybrids: A highly efficient peroxidase-mimetic system for in situ amplified colorimetric immunoassay

Z Gao, M Xu, M Lu, G Chen, D Tang – Biosensors and Bioelectronics, 2015 – Elsevier

2. Experimental. 2.1. Material and reagent. Monoclonal mouse anti-human PSA antibody
(mAb 1 , Abcam, Cambridge, MA), polyclonal goat anti-human PSA antibody (pAb 2 ,
ImmunoReagents Inc., Raleigh, NC), PSA standards (Biocell Biotechnol.

Magnetic bead-based reverse colorimetric immunoassay strategy for sensing biomolecules

Z Gao, M Xu, L Hou, G Chen, D Tang – Analytical chemistry, 2013 – ACS Publications

(Cambridge, MA). Goat polyclonal anti-human PSA antibody (designated as Ab 2 )
was purchased from ImmunoReagents, Inc. (Raleigh, NC). PSA standards were
obtained from Biocell Biotechnol. Co., Ltd. (Zhengzhou, China).

Voltage Dependent Potassium Channel Remodeling in Murine Intestinal Smooth Muscle Hypertrophy Induced by Partial Obstruction

DH Liu, X Huang, X Guo, XM Meng, YS Wu, HL Lu… – PloS one, 2014 – dx.plos.org

Anti-p-Ther antibody (#9386) was obtained from cell signaling technology (City and country of
the company). The secondary antibody DyLight488 conjugated goat anti-mouse IgG
(16117031711) was bought from ImmunoReagents, Inc. (Raleigh, NC, USA).

Magnetic Bead-Based Enzyme-Chromogenic Substrate System for Ultrasensitive Colorimetric Immunoassay Accompanying Cascade Reaction for Enzymatic …

W Lai, D Tang, J Zhuang, G Chen, H Yang – Analytical chemistry, 2014 – ACS Publications

Abcam Inc. (Cambridge, MA). Polyclonal goat antihuman PSA antibody (designated as pAb
2 ) was achieved from ImmunoReagents, Inc. (Raleigh, NC). Squaric acid (SQA) was acquired
from Aladdin (Shanghai, China). 3-Aminopropyltriethoxysilane

Vitamin d assays

JF Zielinski, RJ Olson, MC Mullenix – US Patent App. 13/826,747, 2013 – Google Patents

Wells of a polystyrene microtiter plate were coated with an affinity purified donkey anti-sheep
IgG coating antibody (ImmunoReagents, Inc., Raleigh NC) that specifically binds to an antibody
that specifically binds to both 25OHD 2 and 25OHD 3 . 90 μL of dissociation buffer were

The β‐catenin pathway contributes to the effects of leptin on SREBP‐1c expression in rat hepatic stellate cells and liver fibrosis

X Zhai, K Yan, J Fan, M Niu, Q Zhou… – British journal of …, 2013 – Wiley Online Library

on cultured HSCs, paraformaldehyde-fixed cells were blocked with normal serum and then
incubated with primary antibody against β-catenin (diluted 1:100, Abcam) and a
DyLight488-conjugated secondary antibody (diluted 1:500, ImmunoReagents, Inc. Raleigh, NC,

mTORC1 Is Involved in Hypoxia‐Induced Pulmonary Hypertension Through the Activation of Notch3

W Wang, J Liu, A Ma, R Miao, Y Jin… – Journal of cellular …, 2014 – Wiley Online Library

Physiol. 229: 2117–2125, 2014. © 2014 Wiley Periodicals, Inc. Hypoxia-induced pulmonary
hypertension (HPH) is one of the most common forms of pulmonary hypertension. FITC-conjugated
donkey anti-sheep secondary antibody was from ImmunoReagents (Raleigh, NC).

DNAzyme-functionalized gold–palladium hybrid nanostructures for triple signal amplification of impedimetric immunosensor

L Hou, Z Gao, M Xu, X Cao, X Wu, G Chen… – Biosensors and …, 2014 – Elsevier

(Cambridge, MA). Polyclonal goat anti-human PSA antibody (designated as Ab 2 ) was purchased
from ImmunoReagents, Inc. (Raleigh, NC). PSA standards were obtained from Biocell Biotechnol.
Co., Ltd. (Zhengzhou, China). Hemin was purchased from Tokyo Chem. Inc.

URG4 upregulation is associated with tumor growth and poor survival in epithelial ovarian cancer

W Li, N Zhou – Archives of gynecology and obstetrics, 2012 – Springer

Then, the specimens were briefly washed in PBS and incubated at room temperature with
goat anti-rabbit IgG (GtxRb-003-E, ImmunoReagents, Inc., Raleigh, NC) or goat anti-mouse
IgG (GtxMu-003- F2BIO, ImmunoReagents, Inc., Raleigh, NC). After

Hydrogen sulfide-induced enhancement of gastric fundus smooth muscle tone mediated by voltage-dependent potassium and calcium channels in mice

XM Meng, X Huang, CM Zhang, DH Liu, HL Lu, WX Xu… – wjgnet.com

cells were incubated at room temperature with Alexa Fluor 488-conjugated goat anti-mouse IgG
(1:100, Jackson Immuno Research, West Grove, PA, United States) mixed with Dylight
594-conjugated goat anti-rabbit IgG (1:100, ImmunoReagents Inc, Raleigh, NC, United States

Activin/Nodal Signaling Switches the Terminal Fate of Human Embryonic Stem Cell-derived Trophoblasts

P Sarkar, SM Randall, TS Collier, A Nero… – Journal of Biological …, 2015 – ASBMB

Department of Chemical and Biomolecular Engineering, North Carolina State University, Campus
Box 7905, EB1, Raleigh, North Carolina 27695, Phone Sigma-Aldrich) with 5% BSA (AMRESCO,
Solon, Ohio), 0.1 mg/ml human IgG (Immunoreagents Inc., Raleigh, NC) and 0.3

The methyl donor S-adenosylmethionine potentiates doxorubicin effects on apoptosis of hormone-dependent breast cancer cell lines

CP Ilisso, M Castellano, S Zappavigna, A Lombardi… – Endocrine, 2015 – Springer

All other antibodies were obtained from ImmunoReagents, Inc (Raleigh, NC, USA). CD95-FITC
and FASL were purchased from Gibco (Grand Island, NY). All buffers and solutions were prepared
with ultra-high quality water. All reagents were of the purest commercial grade.

Different regulatory effects of hydrogen sulfide and nitric oxide on gastric motility in mice

X Huang, XM Meng, DH Liu, YS Wu, X Guo… – European journal of …, 2013 – Elsevier

washing, the cells were incubated at room temperature with Alexa Fluor 488-conjugated goat
anti-mouse IgG (1:100, Jackson Immuno Research, West Grove, PA, USA) mixed with DyLight
594-conjugated goat anti-rabbit IgG (1:100, ImmunoReagents Inc, Raleigh, NC, USA) for

Urchin-like (gold CORE)@(platinum shell) nanohybrids: A highly efficient peroxidase-mimetic system for in situ amplified colorimetric immunoassay

Z Gao, M Xu, M Lu, G Chen, D Tang – Biosensors and Bioelectronics, 2015 – Elsevier

2. Experimental. 2.1. Material and reagent. Monoclonal mouse anti-human PSA antibody
(mAb 1 , Abcam, Cambridge, MA), polyclonal goat anti-human PSA antibody (pAb 2 ,
ImmunoReagents Inc., Raleigh, NC), PSA standards (Biocell Biotechnol.

Voltage Dependent Potassium Channel Remodeling in Murine Intestinal Smooth Muscle Hypertrophy Induced by Partial Obstruction

DH Liu, X Huang, X Guo, XM Meng, YS Wu, HL Lu… – PloS one, 2014 – dx.plos.org

Anti-p-Ther antibody (#9386) was obtained from cell signaling technology (City and country of
the company). The secondary antibody DyLight488 conjugated goat anti-mouse IgG
(16117031711) was bought from ImmunoReagents, Inc. (Raleigh, NC, USA).

Magnetic bead-based reverse colorimetric immunoassay strategy for sensing biomolecules

Z Gao, M Xu, L Hou, G Chen, D Tang – Analytical chemistry, 2013 – ACS Publications

(Cambridge, MA). Goat polyclonal anti-human PSA antibody (designated as Ab 2 )
was purchased from ImmunoReagents, Inc. (Raleigh, NC). PSA standards were
obtained from Biocell Biotechnol. Co., Ltd. (Zhengzhou, China).

Magnetic Bead-Based Enzyme-Chromogenic Substrate System for Ultrasensitive Colorimetric Immunoassay Accompanying Cascade Reaction for Enzymatic …

W Lai, D Tang, J Zhuang, G Chen, H Yang – Analytical chemistry, 2014 – ACS Publications

Inc. (Cambridge, MA). Polyclonal goat antihuman PSA antibody (designated as pAb 2 )
was achieved from ImmunoReagents, Inc. (Raleigh, NC). Squaric acid (SQA) was acquired
from Aladdin (Shanghai, China). 3-Aminopropyltriethoxysilane

Vitamin d assays

JF Zielinski, RJ Olson, MC Mullenix – US Patent App. 13/826,747, 2013 – Google Patents

Wells of a polystyrene microtiter plate were coated with an affinity purified donkey anti-sheep
IgG coating antibody (ImmunoReagents, Inc., Raleigh NC) that specifically binds to an antibody
that specifically binds to both 25OHD 2 and 25OHD 3 . 90 μL of dissociation buffer were


ImmunoReagents, Inc. is a leading global manufacturer of quality antibodies and reagents used in pharmaceutical research, life science research and in vitro diagnostics. Product offerings include a wide range of immunochemistry reagents such as purified immunoglobulins, primary antibodies, and secondary antibodies covering a broad spectrum of immunoglobulins from various species. ImmunoReagents also provides custom manufacturing to meet specific customer requirements while adhering to cGMP guidelines and ISO quality systems requirements. The company is located in Raleigh, North Carolina