Antibody Buffers and Formulations
Some common antibody buffers and formulations:
- Phosphate Buffered Saline (PBS)– Dissolve 8.0 grams of NaCl, 0.2 grams of KCl, 1.44 grams of Sodium Phosphate, and 0.24 grams of Potassium Phosphate in 800ml of Deionized (DI) Water. Adjust the pH to 7.2. Bring the final volume to 1 Liter.
- Tris-Buffered Saline (TBS)– Dissolve 8.0 grams of NaCl, 0.2 grams of KCl, 3 grams of Tris base in 800 ml of DI Water. Adjust the pH to 8.0 with 1M Hcl. Adjust the final volume to 1L.
- 10% Sodium Azide- Dissolve 10 grams of Sodium Azide in 100ml of DI Water. IR Tip- Use caution when handling Sodium Azide. It is poisonous.
- ELISA Wash Buffer/Diluent (PBS/0.05% Tween-20)– Add 500µl of Tween-20 detergent to every 1L of PBS.
- ELISA Blocking Buffer (5% Serum/PBS/Tween-20 Solution)– Add 5ml of Serum to every 95ml of PBS/0.05% Tween-20 buffer IR Tip- See IRU’s Blocking Buffer section for additional information.
- Antibody Elution Buffers Low pH (100 mM Citric Acid, pH 4.0)– Add 1.92 grams of Citric Acid to 80ml of DI Water. Adjust the pH to 4.0 using either NaOH of HCl. Adjsut the volume to 100 ml with DI water.
- 1.0 M NaCl– Add 58.44 grams of NaCl to every 1L of DI Water.
- 1.0 M TRIS– pH 6.8- Dissolve 12.1 grams of Tris base in 80ml of DI Water. Adjust the pH to 6.8 with 5M HCL. Adjust the volume to 100 ml with DI water.
- 5N Hydrochloric Acid Solution– Add 59ml of DI Water to a graduated cylinder. Carefully add 41ml of concentrated HCl (37% concentration) to the DI Water. Stir and mix well. CAUTION! Concentrated HCL can cause chemical burns. Use proper safety equipment.
- 5N Sodium Hydroxide Solution– Add 20 grams of granular Sodium Hydroixide and adjust the volume up to 100 ml of DI water. Stir and mix well. CAUTION! Concentrated NaOH can cause chemical burns. Use proper safety equipment.
ImmunoReagents, Inc. is a leading global manufacturer of quality antibodies and reagents used in pharmaceutical research, life science research and in vitro diagnostics. Product offerings include a wide range of immunochemistry reagents such as purified immunoglobulins, primary antibodies, and secondary antibodies covering a broad spectrum of immunoglobulins from various species. ImmunoReagents also provides custom manufacturing to meet specific customer requirements while adhering to cGMP guidelines and ISO quality systems requirements. The company is located in Raleigh, North Carolina