Publications

Publications

WDR79 promotes the proliferation of non-small cell lung cancer cells via USP7-mediated regulation of the Mdm2-p53 pathway

Y Sun, L Cao, X Sheng, J Chen, Y Zhou, C Yang… – Cell Death & Disease, 2017

Bethyl Laboratories, Inc., Montgomery, TX, USA), anti-p53, anti-MDM2 (Santa Cruz) or anti-USP7
(Bethyl Laboratories, Inc.) antibodies at 4 °C overnight, followed by a DyLight 594-conjugated
or DyLight 488-conjugated secondary antibody (ImmunoReagents, Inc., Raleigh, NC

 

Detection of histidine-rich glycoprotein and fibrinogen with nickel-enzyme conjugates: Purification of rabbit HRG

  • Department of Biology, Augsburg College, 2211 Riverside Avenue, Minneapolis, MN, 55454, United States

 

Polyion oligonucleotide-decorated gold nanoparticles with tunable surface charge density for amplified signal output of potentiometric immunosensor

  • a Key Laboratory of Analysis and Detection for Food Safety (MOE & Fujian Province), Collaborative Innovation Center of Detection Technology for Haixi Food Safety and Products (Fujian Province), Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou 350116, PR China
  • b Institute of Environmental and Analytical Science, School of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, PR China
 Received 19 October 2016, Revised 30 December 2016, Accepted 3 February 2017, Available online 20 February 2017

Effect of hypoxia‑inducible factor‑1/vascular endothelial growth factor signaling pathway on spinal cord injury in rats

  • Authors:
    • Hailong Chen
    • Junjie Li
    • Shuhan Liang
    • Bin Lin
    • Qi Peng
    • Peng Zhao
    • Jiawei Cui
    • Yaojian Rao
  • View Affiliations
  • Published online on:Monday, January 16, 2017
  • Pages:861-866 DOI: 10.3892/etm.2017.4049

 

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces hepatic stellate cell (HSC) activation and liver fibrosis in C57BL6 mouse via activating Akt and NF-κB signaling pathways

  • a Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, Nantong, 226001, Jiangsu, PR China
  • b Chuzhou Center for Disease Control and Prevention, Chuzhou, 239499, Anhui, PR China
  • c Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Huangpu, 200011, Shanghai, PR China
 Received 13 January 2017, Revised 9 March 2017, Accepted 10 March 2017, Available online 14 March 2017

DNAzyme-functionalized gold–palladium hybrid nanostructures for triple signal amplification of impedimetric immunosensor

L Hou, Z Gao, M Xu, X Cao, X Wu, G Chen… – Biosensors and …, 2014 – Elsevier

(Cambridge, MA). Polyclonal goat anti-human PSA antibody (designated as Ab 2 ) was purchased
from ImmunoReagents, Inc. (Raleigh, NC). PSA standards were obtained from Biocell Biotechnol.
Co., Ltd. (Zhengzhou, China). Hemin was purchased from Tokyo Chem. Inc.

Stat3 pathway correlates with the roles of leptin in mouse liver fibrosis and sterol regulatory element binding protein-1c expression of rat hepatic stellate cells

W Zhang, M Niu, K Yan, X Zhai, Q Zhou, L Zhang… – The international journal …, 2013 – Elsevier

and activated HSCs (Cassiman et al., 1999), followed by incubation with DyLight594-conjugated
secondary antibody (diluted 1:500, ImmunoReagents, Inc., Raleigh, USA) and DyLight488-
conjugated secondary antibody (diluted 1:500, ImmunoReagents, Inc., Raleigh, USA).

URG4 upregulation is associated with tumor growth and poor survival in epithelial ovarian cancer

W Li, N Zhou – Archives of gynecology and obstetrics, 2012 – Springer

Then, the specimens were briefly washed in PBS and incubated at room temperature with
goat anti-rabbit IgG (GtxRb-003-E, ImmunoReagents, Inc., Raleigh, NC) or goat anti-mouse
IgG (GtxMu-003- F2BIO, ImmunoReagents, Inc., Raleigh, NC). After

Curcumin regulates delta-like homolog 1 expression in activated hepatic stellate cell

J Qiu, Q Zhou, X Zhai, X Jia, Y Zhou – European journal of pharmacology, 2014 – Elsevier

USA) and primary antibody against synaptophysin (SYP, diluted 1:10, Abcam), a marker for
quiescent and activated HSCs (Cassiman et al., 1999), followed by incubation with
DyLight594-conjugated secondary antibody (diluted 1:500, ImmunoReagents, Inc. Raleigh, USA)

GATA binding protein 2 mediates leptin inhibition of PPARγ1 expression in hepatic stellate cells and contributes to hepatic stellate cell activation

Q Zhou, W Guan, H Qiao, Y Cheng, Z Li, X Zhai… – … et Biophysica Acta (BBA …, 2014 – Elsevier

MA, USA), a marker for quiescent and activated HSCs [19], followed by incubation with
DyLight594-conjugated secondary antibody (1:500, ImmunoReagents, Inc., Raleigh, USA) and
DyLight488-conjugated secondary antibody (1:500, ImmunoReagents, Inc., Raleigh, USA).

Activin/Nodal Signaling Switches the Terminal Fate of Human Embryonic Stem Cell-derived Trophoblasts

P Sarkar, SM Randall, TS Collier, A Nero… – Journal of Biological …, 2015 – ASBMB

Cells were blocked in 1X PBS (Sigma-Aldrich) with 5% BSA (AMRESCO, Solon, Ohio), 0.1
mg/ml human IgG (Immunoreagents Inc., Raleigh, NC) and 0.3% Triton X-100, and incubated
overnight with the primary antibody diluted in blocking buffer.

Hydrogen sulfide-induced enhancement of gastric fundus smooth muscle tone mediated by voltage-dependent potassium and calcium channels in mice

XM Meng, X Huang, CM Zhang, DH Liu, HL Lu, WX Xu… – wjgnet.com

the cells were incubated at room temperature with Alexa Fluor 488-conjugated goat anti-mouse
IgG (1:100, Jackson Immuno Research, West Grove, PA, United States) mixed with Dylight
594-conjugated goat anti-rabbit IgG (1:100, ImmunoReagents Inc, Raleigh, NC, United

The methyl donor S-adenosylmethionine potentiates doxorubicin effects on apoptosis of hormone-dependent breast cancer cell lines

CP Ilisso, M Castellano, S Zappavigna, A Lombardi… – Endocrine, 2015 – Springer

All other antibodies were obtained from ImmunoReagents, Inc (Raleigh, NC, USA). CD95-FITC
and FASL were purchased from Gibco (Grand Island, NY). All buffers and solutions were prepared
with ultra-high quality water. All reagents were of the purest commercial grade.

Different regulatory effects of hydrogen sulfide and nitric oxide on gastric motility in mice

X Huang, XM Meng, DH Liu, YS Wu, X Guo… – European journal of …, 2013 – Elsevier

washing, the cells were incubated at room temperature with Alexa Fluor 488-conjugated goat
anti-mouse IgG (1:100, Jackson Immuno Research, West Grove, PA, USA) mixed with DyLight
594-conjugated goat anti-rabbit IgG (1:100, ImmunoReagents Inc, Raleigh, NC, USA) for

Urchin-like (gold CORE)@(platinum shell) nanohybrids: A highly efficient peroxidase-mimetic system for in situ amplified colorimetric immunoassay

Z Gao, M Xu, M Lu, G Chen, D Tang – Biosensors and Bioelectronics, 2015 – Elsevier

2. Experimental. 2.1. Material and reagent. Monoclonal mouse anti-human PSA antibody
(mAb 1 , Abcam, Cambridge, MA), polyclonal goat anti-human PSA antibody (pAb 2 ,
ImmunoReagents Inc., Raleigh, NC), PSA standards (Biocell Biotechnol.

Magnetic bead-based reverse colorimetric immunoassay strategy for sensing biomolecules

Z Gao, M Xu, L Hou, G Chen, D Tang – Analytical chemistry, 2013 – ACS Publications

(Cambridge, MA). Goat polyclonal anti-human PSA antibody (designated as Ab 2 )
was purchased from ImmunoReagents, Inc. (Raleigh, NC). PSA standards were
obtained from Biocell Biotechnol. Co., Ltd. (Zhengzhou, China).

Voltage Dependent Potassium Channel Remodeling in Murine Intestinal Smooth Muscle Hypertrophy Induced by Partial Obstruction

DH Liu, X Huang, X Guo, XM Meng, YS Wu, HL Lu… – PloS one, 2014 – dx.plos.org

Anti-p-Ther antibody (#9386) was obtained from cell signaling technology (City and country of
the company). The secondary antibody DyLight488 conjugated goat anti-mouse IgG
(16117031711) was bought from ImmunoReagents, Inc. (Raleigh, NC, USA).

Magnetic Bead-Based Enzyme-Chromogenic Substrate System for Ultrasensitive Colorimetric Immunoassay Accompanying Cascade Reaction for Enzymatic …

W Lai, D Tang, J Zhuang, G Chen, H Yang – Analytical chemistry, 2014 – ACS Publications

Abcam Inc. (Cambridge, MA). Polyclonal goat antihuman PSA antibody (designated as pAb
2 ) was achieved from ImmunoReagents, Inc. (Raleigh, NC). Squaric acid (SQA) was acquired
from Aladdin (Shanghai, China). 3-Aminopropyltriethoxysilane

Vitamin d assays

JF Zielinski, RJ Olson, MC Mullenix – US Patent App. 13/826,747, 2013 – Google Patents

Wells of a polystyrene microtiter plate were coated with an affinity purified donkey anti-sheep
IgG coating antibody (ImmunoReagents, Inc., Raleigh NC) that specifically binds to an antibody
that specifically binds to both 25OHD 2 and 25OHD 3 . 90 μL of dissociation buffer were

The β‐catenin pathway contributes to the effects of leptin on SREBP‐1c expression in rat hepatic stellate cells and liver fibrosis

X Zhai, K Yan, J Fan, M Niu, Q Zhou… – British journal of …, 2013 – Wiley Online Library

on cultured HSCs, paraformaldehyde-fixed cells were blocked with normal serum and then
incubated with primary antibody against β-catenin (diluted 1:100, Abcam) and a
DyLight488-conjugated secondary antibody (diluted 1:500, ImmunoReagents, Inc. Raleigh, NC,

mTORC1 Is Involved in Hypoxia‐Induced Pulmonary Hypertension Through the Activation of Notch3

W Wang, J Liu, A Ma, R Miao, Y Jin… – Journal of cellular …, 2014 – Wiley Online Library

Physiol. 229: 2117–2125, 2014. © 2014 Wiley Periodicals, Inc. Hypoxia-induced pulmonary
hypertension (HPH) is one of the most common forms of pulmonary hypertension. FITC-conjugated
donkey anti-sheep secondary antibody was from ImmunoReagents (Raleigh, NC).

DNAzyme-functionalized gold–palladium hybrid nanostructures for triple signal amplification of impedimetric immunosensor

L Hou, Z Gao, M Xu, X Cao, X Wu, G Chen… – Biosensors and …, 2014 – Elsevier

(Cambridge, MA). Polyclonal goat anti-human PSA antibody (designated as Ab 2 ) was purchased
from ImmunoReagents, Inc. (Raleigh, NC). PSA standards were obtained from Biocell Biotechnol.
Co., Ltd. (Zhengzhou, China). Hemin was purchased from Tokyo Chem. Inc.

URG4 upregulation is associated with tumor growth and poor survival in epithelial ovarian cancer

W Li, N Zhou – Archives of gynecology and obstetrics, 2012 – Springer

Then, the specimens were briefly washed in PBS and incubated at room temperature with
goat anti-rabbit IgG (GtxRb-003-E, ImmunoReagents, Inc., Raleigh, NC) or goat anti-mouse
IgG (GtxMu-003- F2BIO, ImmunoReagents, Inc., Raleigh, NC). After

Hydrogen sulfide-induced enhancement of gastric fundus smooth muscle tone mediated by voltage-dependent potassium and calcium channels in mice

XM Meng, X Huang, CM Zhang, DH Liu, HL Lu, WX Xu… – wjgnet.com

cells were incubated at room temperature with Alexa Fluor 488-conjugated goat anti-mouse IgG
(1:100, Jackson Immuno Research, West Grove, PA, United States) mixed with Dylight
594-conjugated goat anti-rabbit IgG (1:100, ImmunoReagents Inc, Raleigh, NC, United States

Activin/Nodal Signaling Switches the Terminal Fate of Human Embryonic Stem Cell-derived Trophoblasts

P Sarkar, SM Randall, TS Collier, A Nero… – Journal of Biological …, 2015 – ASBMB

Department of Chemical and Biomolecular Engineering, North Carolina State University, Campus
Box 7905, EB1, Raleigh, North Carolina 27695, Phone Sigma-Aldrich) with 5% BSA (AMRESCO,
Solon, Ohio), 0.1 mg/ml human IgG (Immunoreagents Inc., Raleigh, NC) and 0.3

The methyl donor S-adenosylmethionine potentiates doxorubicin effects on apoptosis of hormone-dependent breast cancer cell lines

CP Ilisso, M Castellano, S Zappavigna, A Lombardi… – Endocrine, 2015 – Springer

All other antibodies were obtained from ImmunoReagents, Inc (Raleigh, NC, USA). CD95-FITC
and FASL were purchased from Gibco (Grand Island, NY). All buffers and solutions were prepared
with ultra-high quality water. All reagents were of the purest commercial grade.

Different regulatory effects of hydrogen sulfide and nitric oxide on gastric motility in mice

X Huang, XM Meng, DH Liu, YS Wu, X Guo… – European journal of …, 2013 – Elsevier

washing, the cells were incubated at room temperature with Alexa Fluor 488-conjugated goat
anti-mouse IgG (1:100, Jackson Immuno Research, West Grove, PA, USA) mixed with DyLight
594-conjugated goat anti-rabbit IgG (1:100, ImmunoReagents Inc, Raleigh, NC, USA) for

Urchin-like (gold CORE)@(platinum shell) nanohybrids: A highly efficient peroxidase-mimetic system for in situ amplified colorimetric immunoassay

Z Gao, M Xu, M Lu, G Chen, D Tang – Biosensors and Bioelectronics, 2015 – Elsevier

2. Experimental. 2.1. Material and reagent. Monoclonal mouse anti-human PSA antibody
(mAb 1 , Abcam, Cambridge, MA), polyclonal goat anti-human PSA antibody (pAb 2 ,
ImmunoReagents Inc., Raleigh, NC), PSA standards (Biocell Biotechnol.

Voltage Dependent Potassium Channel Remodeling in Murine Intestinal Smooth Muscle Hypertrophy Induced by Partial Obstruction

DH Liu, X Huang, X Guo, XM Meng, YS Wu, HL Lu… – PloS one, 2014 – dx.plos.org

Anti-p-Ther antibody (#9386) was obtained from cell signaling technology (City and country of
the company). The secondary antibody DyLight488 conjugated goat anti-mouse IgG
(16117031711) was bought from ImmunoReagents, Inc. (Raleigh, NC, USA).

Magnetic bead-based reverse colorimetric immunoassay strategy for sensing biomolecules

Z Gao, M Xu, L Hou, G Chen, D Tang – Analytical chemistry, 2013 – ACS Publications

(Cambridge, MA). Goat polyclonal anti-human PSA antibody (designated as Ab 2 )
was purchased from ImmunoReagents, Inc. (Raleigh, NC). PSA standards were
obtained from Biocell Biotechnol. Co., Ltd. (Zhengzhou, China).

Magnetic Bead-Based Enzyme-Chromogenic Substrate System for Ultrasensitive Colorimetric Immunoassay Accompanying Cascade Reaction for Enzymatic …

W Lai, D Tang, J Zhuang, G Chen, H Yang – Analytical chemistry, 2014 – ACS Publications

Inc. (Cambridge, MA). Polyclonal goat antihuman PSA antibody (designated as pAb 2 )
was achieved from ImmunoReagents, Inc. (Raleigh, NC). Squaric acid (SQA) was acquired
from Aladdin (Shanghai, China). 3-Aminopropyltriethoxysilane

Vitamin d assays

JF Zielinski, RJ Olson, MC Mullenix – US Patent App. 13/826,747, 2013 – Google Patents

Wells of a polystyrene microtiter plate were coated with an affinity purified donkey anti-sheep
IgG coating antibody (ImmunoReagents, Inc., Raleigh NC) that specifically binds to an antibody
that specifically binds to both 25OHD 2 and 25OHD 3 . 90 μL of dissociation buffer were

Reliable diagnosis of murine type 1 diabetes using a panel of autoantigens and “antigen surrogates” mounted onto a liquid array

Todd M. Doran, Jumpei Morimoto, Scott Simanski, Patrick J. McEnaney and Thomas Kodadek – Mol. BioSyst., 2015, First published online 22 Sep 2015

TBS-T (5 × 5 mL). A 1 : 100 dilution of biotin-conjugated anti-mouse IgG (Immunoreagents, Inc.) in screening buffer was incubated with the library for 1 h at RT and washed with TBS-T (5 × 5 mL). Next, the library was suspended…

TARM1 Is a Novel Leukocyte Receptor Complex–Encoded ITAM Receptor That Costimulates Proinflammatory Cytokine Secretion by Macrophages and Neutrophils

Valeria Radjabova, Piero Mastroeni, Karsten Skjødt, Paola Zaccone, Bernard de Bono, Jane C. Goodall, Edwin R. Chilvers, Jatinder K. Juss, Des C. Jones, John Trowsdale and Alexander David Barrow

Target-Induced Nano-Enzyme Reactor Mediated Hole-Trapping for High-Throughput Immunoassay Based on a Split-Type Photoelectrochemical Detection Strategy

J Zhuang, D Tang, W Lai, M Xu, D Tang – Analytical Chemistry, 2015

antibody (designated as pAb2) were obtained from Abcam Inc. (Cambridge, MA) and ImmunoReagents Inc. (Raleigh, NC), respectively. All high-binding polystyrene 96-well microplates were achieved from Greiner Bio-One (Frickenhausen, 705071, Germany). Gold
S Wei, J Huang, Y Li, J Zhao, Y Luo, X Meng, H Sun… – Journal of Molecular and …, 2015
Secondary, cells were exposed to FITC-and PE-conjugated anti-body (1:200 Immunoreagents, Inc, China) at 37 °C for 2 hours in the dark. Negative control cultures were incubated with isotype IgG instead of the first antibody.
CP Ilisso, L Sapio, DD Cave, M Illiano, A Spina… – Journal of Cellular …, 2015
Blots were incubated with horseradish peroxidase (HRP) conjugated goat anti-rabbit (Immunoreagents Inc., GtxRb- 003-DHRPX) or with HRP-conjugated goat anti-mouse (Immunoreagents Inc., GxMu-003-DHRPX) secondary antibodies.

Protein kinase C modulates transcriptional activation by the juvenile hormone receptor methoprene-tolerant

R Ojani, P Liu, X Fu, J Zhu – Insect Biochemistry and Molecular Biology, 2015
AaTAI antibody. Non-specific goat IgG (ImmunoReagents Inc) was used for mock immunoprecipitation. DNA was purified from the pellets according to the manufacturer’s protocol and analyzed by real-time PCR. For cultured

Rationale: It has been reported that epicardial adipose tissue (EAT) may affect myocardial autonomic function.

Objective: The aim of this study was to explore the relationship between EAT and cardiac sympathetic nerve activity in heart failure (HF) patients.

 

Protective Effect of Tyrosol and S-Adenosylmethionine against Ethanol-Induced Oxidative Stress of Hepg2 Cells Involves Sirtuin 1, P53 and Erk1/2 Signaling

P Stiuso, ML Bagarolo, CP Ilisso, D Vanacore… – International Journal of …, 2016

Diego, CA, USA). Blots were incubated with horseradish peroxidase (HRP)-conjugated
goat anti-rabbit or HRP-conjugated goat anti-mouse (Immunoreagents Inc., Raleigh,
NC, USA) secondary antibodies

Characterization of the lipid envelope of exosome encapsulated HEV particles protected from the immune response

Biochimie, May 5, 2017

Adiponectin down-regulates CREB and inhibits proliferation of A549 lung cancer cells

Pulmonary Pharmacology & Therapeutics, August 2017

CHARACTERIZATION OF THE CHEA2 CHEMOTAXIS OPERON IN AZOSPIRILLUM BRASILENSE

University of Tennessee, May 2017

S-Adenosylmethionine-mediated apoptosis is potentiated by autophagy inhibition induced by chloroquine in human breast cancer cells

Journal of Cellular Physiology, June 2017

Vesicular stomatitis virus pseudotyped with Ebola virus glycoprotein serves as a protective,

non-infectious vaccine against Ebola virus challenge in mice 

Journal of Virology, June 2017

Effect of Class II HDAC inhibition on glutamate transporter expression and survival in SOD1-ALS mice

Neuroscience Letters, July 2017